infant foreskin Search Results


unaffected human infant foreskin fibroblasts (coriell)  (Coriell Institute for Medical Research)
90
Coriell Institute for Medical Research unaffected human infant foreskin fibroblasts (coriell)
Changes in nuclear architecture as HGPS cells age in culture. Typical nuclei from HGPS cells in passages 6 (a), 13 (b), and 26 (c), normal aged <t>human</t> AG09602B (d), and <t>foreskin</t> fibroblast (e) controls after labeling with LA Ab. Nuclei in control cells appeared similar from passage 6 through passage 17. The lamina increased in prominence or thickness in HGPS cell nuclei by passage 26 (c). Earlier passage HGPS (a) and control cell nuclei (d and e) were normal in appearance. Passage 6 and 26 HGPS cells were double labeled with LA (g and j) and LB (h and k) Abs. Note the extensive coincidence in the staining patterns in the merged image at passage 6 (i) and the decrease in this coincidence in a cell by passage 26 (l). The table shows that the contour ratio decreased as a function of passage number in HGADFN003 cells. P values were calculated relative to passage 2 of the AG09602B <t>fibroblasts.</t> There was no significant change in the contour ratio in control cells between passages 2 and 17. In HGADFN003 whole-cell extracts immunoblotted with LA/C Abs, a band migrating between LA (upper band, *) and lamin C (lower band, *) was observed and became more prominent as the passage number increased (f). (Scale bars = 5 μm.)
Unaffected Human Infant Foreskin Fibroblasts (Coriell), supplied by Coriell Institute for Medical Research, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/unaffected human infant foreskin fibroblasts (coriell)/product/Coriell Institute for Medical Research
Average 90 stars, based on 1 article reviews
unaffected human infant foreskin fibroblasts (coriell) - by Bioz Stars, 2026-03
90/100 stars
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normal human epidermal keratinocytes from infant foreskin  (PROVITRO GmbH)
90
PROVITRO GmbH normal human epidermal keratinocytes from infant foreskin
Changes in nuclear architecture as HGPS cells age in culture. Typical nuclei from HGPS cells in passages 6 (a), 13 (b), and 26 (c), normal aged <t>human</t> AG09602B (d), and <t>foreskin</t> fibroblast (e) controls after labeling with LA Ab. Nuclei in control cells appeared similar from passage 6 through passage 17. The lamina increased in prominence or thickness in HGPS cell nuclei by passage 26 (c). Earlier passage HGPS (a) and control cell nuclei (d and e) were normal in appearance. Passage 6 and 26 HGPS cells were double labeled with LA (g and j) and LB (h and k) Abs. Note the extensive coincidence in the staining patterns in the merged image at passage 6 (i) and the decrease in this coincidence in a cell by passage 26 (l). The table shows that the contour ratio decreased as a function of passage number in HGADFN003 cells. P values were calculated relative to passage 2 of the AG09602B <t>fibroblasts.</t> There was no significant change in the contour ratio in control cells between passages 2 and 17. In HGADFN003 whole-cell extracts immunoblotted with LA/C Abs, a band migrating between LA (upper band, *) and lamin C (lower band, *) was observed and became more prominent as the passage number increased (f). (Scale bars = 5 μm.)
Normal Human Epidermal Keratinocytes From Infant Foreskin, supplied by PROVITRO GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/normal human epidermal keratinocytes from infant foreskin/product/PROVITRO GmbH
Average 90 stars, based on 1 article reviews
normal human epidermal keratinocytes from infant foreskin - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier

Image Search Results


Changes in nuclear architecture as HGPS cells age in culture. Typical nuclei from HGPS cells in passages 6 (a), 13 (b), and 26 (c), normal aged human AG09602B (d), and foreskin fibroblast (e) controls after labeling with LA Ab. Nuclei in control cells appeared similar from passage 6 through passage 17. The lamina increased in prominence or thickness in HGPS cell nuclei by passage 26 (c). Earlier passage HGPS (a) and control cell nuclei (d and e) were normal in appearance. Passage 6 and 26 HGPS cells were double labeled with LA (g and j) and LB (h and k) Abs. Note the extensive coincidence in the staining patterns in the merged image at passage 6 (i) and the decrease in this coincidence in a cell by passage 26 (l). The table shows that the contour ratio decreased as a function of passage number in HGADFN003 cells. P values were calculated relative to passage 2 of the AG09602B fibroblasts. There was no significant change in the contour ratio in control cells between passages 2 and 17. In HGADFN003 whole-cell extracts immunoblotted with LA/C Abs, a band migrating between LA (upper band, *) and lamin C (lower band, *) was observed and became more prominent as the passage number increased (f). (Scale bars = 5 μm.)

Journal:

Article Title: Accumulation of mutant lamin A causes progressive changes in nuclear architecture in Hutchinson-Gilford progeria syndrome

doi: 10.1073/pnas.0402943101

Figure Lengend Snippet: Changes in nuclear architecture as HGPS cells age in culture. Typical nuclei from HGPS cells in passages 6 (a), 13 (b), and 26 (c), normal aged human AG09602B (d), and foreskin fibroblast (e) controls after labeling with LA Ab. Nuclei in control cells appeared similar from passage 6 through passage 17. The lamina increased in prominence or thickness in HGPS cell nuclei by passage 26 (c). Earlier passage HGPS (a) and control cell nuclei (d and e) were normal in appearance. Passage 6 and 26 HGPS cells were double labeled with LA (g and j) and LB (h and k) Abs. Note the extensive coincidence in the staining patterns in the merged image at passage 6 (i) and the decrease in this coincidence in a cell by passage 26 (l). The table shows that the contour ratio decreased as a function of passage number in HGADFN003 cells. P values were calculated relative to passage 2 of the AG09602B fibroblasts. There was no significant change in the contour ratio in control cells between passages 2 and 17. In HGADFN003 whole-cell extracts immunoblotted with LA/C Abs, a band migrating between LA (upper band, *) and lamin C (lower band, *) was observed and became more prominent as the passage number increased (f). (Scale bars = 5 μm.)

Article Snippet: Fibroblasts from unaffected older adults (AG09602B derived from a 92-yr-old female; Coriell) and unaffected human infant foreskin fibroblasts (Coriell) were used as controls.

Techniques: Labeling, Staining

Pre-LA accumulation in the nuclei of HGPS cells. In passage 6 and 13 HGPS cells, the pre-LA pattern consisted of weakly staining nucleoplasmic foci (a and b) similar to control AG09602B fibroblasts (d). However, by passage 26, the pre-LA staining was much more intense in the lobulated nuclei and was primarily associated with the lamina region (c). (e–g) Electron microscopic observations of passage 26 HGPS HGADFN003 cells (e and f) and normal human foreskin fibroblasts (g). A high-magnification view of the nuclear envelope in a normal human foreskin fibroblast showed a normal array of heterochromatin adjacent to the nuclear envelope, making any lamina structure difficult to detect (g). A low-magnification view of a passage 26 HGADFN003 nucleus showed extensive lobulation (e). A higher-magnification view of a passage 26 cell showed a loss of peripheral heterochromatin and a prominent electron-dense lamina region associated with the inner nuclear envelope membrane (f). In f and g, the nucleus is to the left. [Scale bars = 5 μm (a–e) and 200 nm (f and g).]

Journal:

Article Title: Accumulation of mutant lamin A causes progressive changes in nuclear architecture in Hutchinson-Gilford progeria syndrome

doi: 10.1073/pnas.0402943101

Figure Lengend Snippet: Pre-LA accumulation in the nuclei of HGPS cells. In passage 6 and 13 HGPS cells, the pre-LA pattern consisted of weakly staining nucleoplasmic foci (a and b) similar to control AG09602B fibroblasts (d). However, by passage 26, the pre-LA staining was much more intense in the lobulated nuclei and was primarily associated with the lamina region (c). (e–g) Electron microscopic observations of passage 26 HGPS HGADFN003 cells (e and f) and normal human foreskin fibroblasts (g). A high-magnification view of the nuclear envelope in a normal human foreskin fibroblast showed a normal array of heterochromatin adjacent to the nuclear envelope, making any lamina structure difficult to detect (g). A low-magnification view of a passage 26 HGADFN003 nucleus showed extensive lobulation (e). A higher-magnification view of a passage 26 cell showed a loss of peripheral heterochromatin and a prominent electron-dense lamina region associated with the inner nuclear envelope membrane (f). In f and g, the nucleus is to the left. [Scale bars = 5 μm (a–e) and 200 nm (f and g).]

Article Snippet: Fibroblasts from unaffected older adults (AG09602B derived from a 92-yr-old female; Coriell) and unaffected human infant foreskin fibroblasts (Coriell) were used as controls.

Techniques: Staining